Humanized Mouse Models

The use of humanized mice as in vivo models has become commonplace in studies for immuno-oncology and infectious disease research. They have also improved the ability to predict human responses to new drugs being clinically developed.

Depending on research requirements, it is critical that researchers have the ability to obtain study-ready animals or maintain the option to humanize the mice in-house to meet their project timelines.

Choose the Humanized Mouse Model Option
That's Best for You

humanized mouse in the hands of a researcherWhen your research requires humanized animal models, Charles River offers two options, both founded on the NCG triple-immunodeficient mouse.

 

Study-Ready* Model    NCG/PBMC Humanization Kit
*NCG mice shipped already injected with PBMC cells are available in North America only.

Advance Your Program with Humanized Mouse Models

Currently, the Charles River NCG triple-immunodeficient mouse model is the foundation for our humanization portfolio. Its unique phenotype allows it to host xenograft cells, tissue, and human immune system components. It displays noticeable improvements of foreign tissue transplantation and engraftment, compared to previous generations of immunocompromised mice.

The NCG mouse is best suited for studies on tumor biology, immuno-oncology, infectious diseases, graft-versus-host disease (GvHD), hematopoiesis, and tissue transplantation.

Charles River's expertise in oncology drug discovery, spanning from target identification to IND studies, makes us ideally suited to run your studies for you. Learn more about our humanized mouse tumor model studies.

Are humanized mice the emerging model
to evaluate PD-1 therapeutics?

humanized miceCheck out our scientific poster, Validation of Humanized PD-1 Knock-in Mice as an Emerging Model to Evaluate Human-Specific PD-1 Therapeutics, to learn more.
 

Download Poster

Study-Ready HuPBMC-NCG Mouse Models

Immunodeficient mouse models engrafted with human peripheral blood mononuclear cells (PBMCs) are available for short-term studies. In our hands, engrafted human cells can be detected as early as 10 days after injection of the PBMCs. A good proportion of these adult immune cells are of human T-cell origin (CD4+ and CD8+).

The ability of human immune cells to engraft in the NCG mouse makes it a powerful tool to evaluate compounds for immuno-oncology, GvHD, infectious diseases, and inflammatory diseases.


Choose the option that's best for you:

*NCG mice shipped already injected with PBMC cells are available in North America only.
 

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Frequently Asked Questions (FAQs) About Humanized Mice

  • What are humanized mice used for?

    Humanized mouse models are used to compare tumor therapies, study the human immune system, and to study the function of genes in healthy and diseased models.

  • What are the benefits of the NCG humanized mouse model?

    The HuPBMC-NCG mouse is a convenient, ready-to-use model that is pre-injected and tested for effective engraftment in the NCG mouse model. Pre-screened PBMCs save time, labor, and costs associated with donor qualification. The PBMC cell inventory has been screened for engraftment rate, body weight loss, and study term.

  • How can I create humanized mouse models?

    Create your own humanized mouse models on your own timeline. The NCG/PBMC Humanization Kit allows researchers to avoid additional work needed to qualify PBMC donor cells. By providing a "kit" of both cells and mice, this puts the researcher in control of study timing, which is a distinct advantage when dealing with a limited therapeutic window.

    • Study-ready: Cells are pre-tested for engraftment in the NCG mouse model.
    • Efficient: Cells have been pre-screened, eliminating time, labor, and cost.
    • Flexible: Can be humanized according to study timelines.
    • Trusted Source: This kit is a product of a partnership between Charles River and HemaCare®.

     

  • How are humanized mouse models created?

    The NCG model's immune system is humanized by irradiating and destroying the host immune system. Once the immune system is knocked out, human PBMCs are injected to create a humanized model. Engrafted human cells can be detected as early as 10 days after the PBMC injection.

    Humanized Mice

    Humanized mouse models can be used for tumor models to screen cancer therapies in an intact human immune system.
    Schematic of humanized mouse tumor models. The mouse immune systems are humanized by irradiating and destroying the host immune system and introducing human immune cells.