传统的微生物检测方法在很大程度上依赖漫长的培养期来实现可见的生长，菌落要生长得足够大才能在微生物限度内进行可信计数，或者在无菌检测中明确浊度。基于 ATP 生物发光的 Celsis®快速检测技术可将这些培养期缩短 50%。
Celsis® ATP 生物发光反应概述
Celsis® 试剂的基本原理虽然简单，但功能强大。Celsis® 快速微生物检测试剂技术基于天然存在的 ATP 生物发光反应，该技术也可提供萤火虫的特征光。
该反应称为荧光素 - 荧光素酶反应，当荧光素酶将荧光素转化为氧化荧光素从而发光时，就会发生这种反应。只有存在 ATP 时才会发生这种反应。由于 ATP 存在于包括原核生物的所有活细胞中，如果样品在正常条件下不含 ATP，则可用作污染的标记物。
What is the ATP-bioluminescence assay used for?
The ATP bioluminescence can be used as a screening assay for the presence of adenosine triphosphate (ATP) present in a sample when no ATP is present. Since ATP is present in all living cells (including those of bacteria, yeasts, and molds), it can be used in microbial testing to detect the presence of contamination in a sample.
Why is ATP-bioluminescence a gold standard detection method?
ATP-bioluminescence, utilizing the luciferase assay, is considered a gold standard detection due to its highly efficient ability to detect microbial contamination at extremely small concentrations. When this reaction is controlled and allowed to exponentially react using the timed injection of specialized reagents, the reaction provides positive confirmation of contamination.
How long has ATP-bioluminescence been used for testing?
ATP-bioluminescence, with numerous scientific publications studying its properties, has been determined a means for microbial detection since 1989. The ATP-bioluminescence assay used in Celsis AMPiScreen® reagents was first characterized in 1955, when it was known as the adenylate kinase assay at the time. It has been described in microbial testing since 1998.