借助 AccuGENX-ID® FunITS 进行真菌鉴定

长期以来,真菌鉴定,特别是丝状真菌,一直是非常困难的任务。由于精确鉴定物种等级的丝状真菌需要丰富经验和大量时间,通常的做法是将这些生物鉴定到属的水平,或者在某些情况下,简单地将其鉴定为“霉菌”。

真菌鉴定的遗传方法为物种等级鉴定提供了更可靠、更快速的方法。FunITS 使用内部转录间隔区 (ITS2),这是被真菌分类学家广泛接受为“黄金标准”的常见真菌 DNA 区域。由于 ITS 区在密切相关的物种之间具有比其他 DNA 区(如 D2)更高的变异程度,因此 “ITS” 可以说是鉴定真菌物种的最佳方法。

AccuGENX-ID® FunITS 基因型分析过程遵循分类学家使用的参考方法,因为它为鉴定提供最准确、最可靠的序列数据。如果将此流程与全覆盖、已验证的库相结合,由此得出的系统发育分析在业内是无以伦比的。

  • Order Codes
    Turnaround Time (TAT) Test Code
    Same day FunITS-0
    1 day FunITS-1
    2 days FunITS-2
    5 days FunITS-5

Frequently Asked Questions (FAQs ): Fungal Identification Services

  • What techniques are used to identify fungal isolates?

    Comparative DNA sequencing of ITS and D2 rRNA regions are the two main options for identifying fungal isolates. Accugenix® fungal identification service uses the ITS2 sequence, which has proven to be the most widely accepted DNA sequence for fungi. ITS2 has more variability than the D2 expansion segment DNA sequence, thus providing greater specificity. This yields a much higher resolution in species identification than D2. ITS2 is recognized for the identification of fungal species via DNA sequence signatures by the International Barcode of Life Consortium.

  • Why is an accurate fungal identification important?

    Accurate fungal identification is critical for environmental monitoring (EM) programs in pharmaceutical and other regulated product manufacturing industries. Identification of unknown isolates is an essential first step in understanding the risk that microorganisms have on the manufacturing environment, final product, and subsequently the patients. Accurate fungal identification services that provide EM data tracking and trending solutions maintain a clear baseline view of the facility’s microbial flora so that unusual activity is recognized early and there is time for remediation.

  • What are the critical elements of accurate fungal identification?

    The organism reference library is an essential part of the fungal identification process. A fungal isolate cannot be accurately identified without a matching species entry in the database; therefore, a relevant and validated reference library is equally as important as the technology and methodology used to identify fungi. Aside from library comparison, interpretation of the sequence and report generation are also critical parts of the fungal identification process.