菌株分型法

与使用 16S 和 ITS2 基因鉴定细菌和真菌的 AccuGENX-ID® 方法一样,AccuGENX-ST® 序列分型采用标准分子生物学方法进行 DNA 提取,PCR 扩增和蛋白质编码靶基因的 DNA 测序,以表征分离物的菌株水平。将得到的 DNA 序列或序列类型与数据库中的其他序列或序列类型进行比较,并在进化树树中显示关系。Charles River 将根据要求对任何样品或样品组进行比较,并且您无需支付额外费用。

RiboPrinter® 利用限制酶和基于片段的分析提供细菌表征或菌株分型,以确定污染源或菌株相关性。

  • How do you select the right strain typing technology?

    There are a number of different technologies that will compare isolates of the same species down to the strain level. Whether just a few genes should be sequenced or the entire genome, it can be challenging to figure out exactly what you need to achieve your desired outcome. Our experts are available to discuss your specific situation and recommend the appropriate test.

  • Can ribotyping be used for fungal isolates?

    Ribotyping is only applicable for bacteria while AccuGENX-ST® strain typing can process both fungal and bacterial samples from our validated method list. If the organism of interest is not on our list, our scientists can develop and validate a new method in 3-4 weeks for an additional fee. 

  • What is the difference between a microbial species and a strain?

    A species is a group of strains that are genetically very similar, whereas strains have unique genetic sequences. Taxonomists use type strains to represent and define the DNA sequence of a species, but in actuality there is genetic diversity within a species, which are called strains. Learn more about the bacterial species concept.