RiboPrinter®系统将 RiboPrint®图案处于固定相似度范围内的样品组合在一起。这些图案可以在彼此之前或之后生成，并用于确定作为跟踪和趋势化项目一部分的分离物之间的相似性水平。我们为每个提交核糖体分型样本的客户构建和维护自定义库。每次客户提交新样本时，都会将其与自定义库中的所有其他先前样本进行比较，并分配给 RiboGroup。
在某些情况下，核糖体分型无效，因为有些物种在 RiboPrinter® 分析的核糖体 RNA 操纵子中几乎没有多样性。在这些情况下，菌株分型和表征（SLST 和 MLST）的基于序列的方法可以提供比自动核糖体分型更高水平的鉴别和可重复性，并应受到格外关注。
Frequently Asked Questions
What is ribotyping of bacterial strains?
Ribotyping is an automated fragment-based analysis of the organism’s DNA fingerprint using the RiboPrinter® system for bacterial characterization.
What is a riboprinting pattern?
Riboprinting is a fragment-based technology that utilizes restriction enzymes to target and cut regions of the ribosomal RNA genes (5S, 16S, 23S, and the spacer region including Glu-tRNA), generating a DNA fingerprint that is unique to the organism at the strain level. This DNA fingerprint is known as a riboprinting pattern.
Are more modern and accurate technologies available for bacterial characterization?
Sequence-based methods of strain typing such as single or multilocus sequence typing (SLST and MSLT) are the recommended options for a higher level of discrimination and repeatability. Most academic research sources, such as the American Society for Microbiology’s Journal of Clinical Microbiology, agree that MLST is the most powerful method for discriminating between isolates.
What is the difference between riboprinting and strain typing services?
The AccuGENX-ST® strain typing service performs DNA sequencing of targets outside of the standard regions sequenced for microbial identification such as 16S, D2, or ITS2, to show differences and similarities of the strain. This method is superior to riboprinting, although ribotyping can be used without any prior development. Strain typing can offer a definitive answer when tracking sources of contamination or verifying production strains.