rCR vials and cartridges for standardized endotoxin testing

As more labs adopt recombinant cascade reagent (rCR) methods for bacterial endotoxin testing, it’s critical to build processes that protect compliance and deliver reliable results. Whether you are just beginning or refining established workflows, this guide outlines proven steps to help you succeed. 

 You’ll learn how to: 

  • Compare rCR methods with traditional LAL approaches and understand when each is appropriate
  • Identify compatible instruments and software to support your BET
  • Select the right accessories and consumables to minimize error and improve reproducibility
  • Apply best practices for assay setup, handling, and execution to reduce variability and invalid results
  • Conduct comparability studies to ensure equivalency when transitioning from LAL to rCR  

Endosafe® Trillium™ vials and cartridges replicate the LAL enzymatic cascade through recombinant proteins. This approach reduces reliance on animal-derived reagents while maintaining the accuracy and robustness required for GMP testing. When paired with our instruments and software, rCR methods simplify training, minimize analyst variability, and reduce retest rates that drive up operational costs. 

By following the recommendations in this guide, you can shorten time to result, strengthen data integrity, and scale rCR adoption across your lab with confidence. 

Fill out the form to receive the full guide directly in your inbox. 

Using the Endosafe® Trillium™ rCR cartridges alongside our routine testing was straightforward for our technicians, as the test method remains identical, and the results are reported the same way. We were pleased with the technology implementation, quality of data, and onboarding as it was simple and seamless, which is ideal when introducing such an impactful change in the microbiology lab. We look forward to moving to Endosafe® Trillium™ rCR cartridges for routine testing later in the year as part of our environmental sustainability efforts.”

Greg White, Microbiology Laboratory Supervisor & Blackpool STEM Lead, DePuy Synthes International