CryoStor® Cell Freezing Media from Charles River
CryoStor® cell freezing media comes pre-formulated with dimethyl sulfoxide (DMSO), providing a secure and protective environment for cells and tissues during the entire process of freezing, storage, and thawing. This also aids in managing the molecular reaction to cryopreservation, resulting in improved cell viability and functionality without relying on serum, proteins, or excessive amounts of cytotoxic agents.
- Ready to use
- Protein-free and serum-free
- CGMP-manufactured
- USP/high quality components
- Meets USP sterility and USP endotoxin testing standards
- FDA master file
Explore CryoStor® Freezing Media Products for Cells and Tissues
CryoStor® CS10 Freeze Media
CryoStor CS10 freeze media is a ready-to-use product that is pre-formulated with 10% DMSO and requires no additional preparation. Conveniently offered in a 10 mL vial size and 100 mL vial or bag, CryoStor CS10 is serum-free, protein-free, and manufactured following CGMP standards.
| Size | Product Code |
| CS10 10 mL vial | BPM 210373 |
| CS10 100 mL bottle | BPM 210102 |
| CS10 100 mL bag | BPM 210202 |
CryoStor® CS2 Freeze Media
CryoStor CS2 freeze media is a ready-to-use, proprietary freeze media for cell and tissue preservation at temperatures between -70 and -196°C. Offered in a 100 mL bottle, it contains pre-formulated 2% USP-grade DMSO, a cryoprotective agent that helps prevent damage from ice formation. CryoStor CS2 freeze media is serum-free, protein-free, and manufactured following CGMP standards.
| Size | Product Code |
| CS2 100 mL bottle | BPM 202102 |
CryoStor® CS5 Freeze Media
CryoStor CS5 freeze media is offered in two convenient forms, 100 mL bags or bottles. It is composed of pre-formulated 5% USP-grade DMSO, to safeguard cells and tissues during freezing, storage, and thawing. Manufactured following CGMP guidelines, CryoStor CS5 freeze media is produced without serum or protein.
| Size | Product Code |
| CS5 100 mL bottle | BPM 205102 |
| CS5 100 mL bag | BPM 205202 |
Frequently Asked Questions About CryoStor® Freezing Media
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Can I use CryoStor freeze media in my manufacturing runs for clinical use?
Yes. The CryoStor CS10, CS5, and CS2 freeze media products we offer can be used in clinical manufacturing runs. They are produced under CGMP guidelines in a controlled environment, making them sterile and toxin-free.
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Do CryoStor CS10, CS5, or CS2 freeze media products offered by Charles River contain any serum, protein, or animal-derived components?
No. The range of CryoStor freeze media products we offer, including CS10, CS5, and CS2, are all serum-, protein-, and animal-free.
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Do you have data supporting stability of cells using CryoStor cell freezing media?
Yes. Our white paper, A Comparative Study: Are Cryopreserved Leukopaks a Viable Alternative to Fresh?, contains data supporting the stability of our leukopaks cryopreserved with CryoStor freeze media.
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What are the differences among CryoStor CS10, CS5, and CS2 freeze media products?
The main difference among the range of CryoStor cell freezing media we offer is the percentage of DMSO (dimethyl sulfoxide) used. CryoStor CS10 contains 10%, CryoStor CS5 contains 5%, and CryoStor CS2 contains 2%. Choose the best option for your needs to ensure the optimal preservation of your cells.
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How does DMSO work as a cryoprotectant in CryoStor cell freezing media, and what advantages does it offer over other cryoprotectants?
DMSO, also known as dimethyl sulfoxide, is a highly recommended cryoprotectant for preserving cell viability during the freezing process. Its unique ability to penetrate cell membranes and inhibit formation of damaging ice crystal while also effectively reducing the freezing point of the surrounding solution, ensures the survival of cells upon thawing. CryoStor cell freezing media products are pre-formulated with DMSO, so that your cells maintain their viability. DSMO is a crucial element in any dependable cell freezing medium.
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