Why Buy Cell Freezing Media?
Cell freezing media from Charles River significantly extends stability and improves post-preservation viability and function of your biologic source material, intermediates, and final products.
- Ready to use
- Protein-free and serum-free
- CGMP-manufactured
- USP/high quality components
- Meets USP sterility and USP endotoxin testing standards
- FDA master file
Explore Cell Freezing Media and Biopreservation Media Products
| CryoStor® | CryoStor® proprietary cell freezing media products are pre-formulated with USP-grade DMSO and are intended for cryopreservation of biological materials at -70 °C to -196 °C. Explore CryoStor® cell freezing media |
| HypoThermosol® | HypoThermosol® FRS biopreservation media is designed to provide maximum storage and shipping stability for biologics at 2 °C to 8 °C. Explore HypoThermosol® FRS biopreservation media |
Frequently Asked Questions About Cell Freezing Media
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Can I use cell freezing media and biopreservation media products in my manufacturing runs for clinical use?
Yes. Cell freezing media and biopreservation media products are manufactured in accordance with CGMP guidelines in a regulated setting, ensuring they are sterile and free from toxins. This process guarantees that each batch meets consistent high-quality standards, which is critical for reproducibility in research and clinical applications.
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Do cell freezing media and biopreservation media products contain any, serum, protein, or animal-derived components?
No. The range of CryoStor freeze media products we offer, including CS10, CS5, and CS2, are all serum-, protein-, and animal-free.
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Is there data supporting the stability of cells using CryoStor cell freezing media?
Yes. We have data supporting the stability of cells using CryoStor cell freezing media in our white paper, A Comparative Study: Are Cryopreserved Leukopaks a Viable Alternative to Fresh?
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Is DMSO cytotoxic, and if so, how can the cytotoxic effects be mitigated during the freezing and thawing processes?
When it comes to cell freezing media, DMSO (dimethyl sulfoxide) is a commonly used cryoprotectant. While it may have some toxicity at high concentrations and when cells are exposed to it for long periods of time prior to freezing, when used appropriately, it is considered relatively safe for cells as compared to other cryoprotectants. After cryopreservation, however, thorough washing is necessary to remove any remaining DMSO before the cells can be used.
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Why is DMSO considered a good cryoprotectant for cell freezing media products?
When it comes to preserving cell viability during the freezing process, DMSO (dimethyl sulfoxide) is an excellent choice as a cryoprotectant in cell freezing media. Its unique abilities to penetrate cell membranes and disrupt the formation of damaging ice crystals while also lowering the freezing point of the solution surrounding the cells, ensure their survival after thawing. DSMO is a crucial element for any dependable cell freezing media product.
The cell freezing media products we offer are pre-formulated with DMSO, in your choice of three formulations.
References
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References
Novel cryoprotectant significantly improves the post-thaw recovery and quality of HSC from CB
Stylianou J, Vowels M, Hadfield K.Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type
Ivanics T, Bergquist JR, Liu G, Kim MP, Kang Y, Katz MH, Perez MVR, Thomas RM, Fleming JB, Truty MJManufacturing of CD34 + HPC-enriched, high-purity mononuclear cell products from umbilical cord blood
Wynn L, Wilson MG, Leonforte C.Non-toxic freezing media to retain the stem cell reserves in adipose tissues
Shaik S, Wu X, Gimble JM, Devireddy R.Cryopreservation of adipose-derived stromal/stem cells using 1-2% Me(2)SO (DMSO) in combination with pentaisomaltose: An effective and less toxic alternative to comparable freezing media
Svalgaard JD, Munthe-Fog L, Ballesteros OR, Brooks PT, Rangatchew F, Vester-Glowinski PV, Haastrup EK, Fischer-Nielsen A.