1H-MRS and 31P-MRS in CNS Drug Development
Charles River has implemented pulse sequences for localized 1H-MRS, enabling quantitative determination of 15-18 metabolite concentrations that is detected using LCModel software. Our imaging team offers 31P MRS to detect changes in energy metabolism including ATP, phosphocreatine, and inorganic phosphate.
Image gallery: 1H-MRS in aged female Fisher rats and Alzheimer’s disease mouse models. In both models, N-acetyl aspartate and Glutamate are decreased while myo-inositol is increased.
MRS Frequently Asked Questions (FAQs)
How can MRS be combined with biochemical assays?
MRS studies are easily combined with established biochemical assays. Since MRS is noninvasive, data from multiple time points can be collected prior to endpoint sampling that can be used for relevant biomarker analysis.
What species are you able to perform MRI on?
Currently at the Charles River site in Kuopio, Finland, MRI imaging is performed on rats and mice and the Charles River site in Sherbrooke, Canada can image dogs and non-human primates.
Which software does Charles River use for brain volumetric analysis?
Currently, we use MATLAB based software that was developed in-house and for brain volumetry analysis, the freely available IDIS software package is used.