Why Buy High Quality CD3+ Pan T Cells from Charles River?
We perform rigorous donor screening and product QC testing to ensure consistent CD3+ T cell lots for discovery and therapeutic development.
Features of Our CD3+ Pan T Cells
- Guaranteed post-thaw viability ≥ 90% as tested by NucleoCounter NC-200™
- Guaranteed post-thaw purity ≥ 90% as tested via flow cytometry
- Guaranteed CD3+ T cell yields post thaw as listed per vial size
- Available using positive or negative immunomagnetic cell selection options
- Available in 5M, 10M, 25M, and 50M vial sizes from inventory. Full leukopak kit productions, custom formats and aliquot sizes are also available.
- Fill volume is 1ml of CD3+ T cells in a 2ml vial size
- Full donor screening (serology and infectious disease panel)
- Isolated from fresh leukopaks that are collected from healthy human donors that have consented under an IRB approved protocol
Buy CD3+ T Cells that Best Suit Your Project Needs
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CD3+ Pan T Cells
CD3+ pan T cells contain a mix of all the T cell populations that express the CD3 surface biomarker including helper T cells, cytotoxic T cells, gamma/delta T cells, regulatory T cells (Tregs), and natural killer T cells (NKTs).
Cell & Isolation Type Size Product Code CD3+ Pan T Cells
(Positive Selection)5 x 10e6 cells PB03C-1 10 x 10e6 cells PB03C-2 25 x 10e6 cells PB03C-3 50 x 10e6 cells PB03C-4 Custom PB03C-KIT* Full leukopak isolation PB03C-FL-KIT* CD3+ Pan T Cells
(Negative Selection)5 x 10e6 cells PB03NC-1 10 x 10e6 cells PB03NC-2 25 x 10e6 cells PB03NC-3 50 x 10e6 cells PB03NC-4 Custom PB03NC-KIT* Full leukopak isolation PB03NC-FL-KIT* Contact us if you don’t see a specific product, size, or format, or would like to customize your request.
* Product is made to order.Your company or academic institution may be eligible for pricing discounts. Government labs located in the United States are also eligible for GSA MAS discounts. Request a quote to find out more.
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Naive CD3+ T Cells
Naive CD3+ T cells are considered immature T cells that have not yet been activated by specific antigens.
Naive CD3+ T Cells
(Positive Selection)10 x 10e6 cells PB0345RAC-1* Naive CD3+ T Cells
(Negative Selection)10 x 10e6 cells
Full leukopak isolationPB0345RANC-1*
PB0345RANC-FL-KIT*Contact us if you don’t see a specific product, size, or format, or would like to customize your request.
* Product is made to order.Your company or academic institution may be eligible for pricing discounts. Government labs located in the United States are also eligible for GSA MAS discounts. Request a quote to find out more.
What are CD3+ T Cells and How are They Used in Research?
CD3 is the primary T cell surface biomarker that is critical for the signaling cascade activating T cells and is used to distinguish T cells from other immune cells. The term “CD3+ pan T cells” refers to the mix of all T cell subpopulations that express the CD3 biomarker, including helper T cells, cytotoxic T cells, gamma/delta T cells, regulatory T cells (Tregs), and natural killer T cells (NKTs).
CD3+ T cells are used in a wide range of immunology applications including:
- Cancer therapies
- Vaccine development
- Immune cell therapies
- Infectious disease studies
- Immune assay validation/development
- CAR-T therapy,
- Mixed lymphocyte reaction assays
Lineage of CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs)
Receive a complimentary poster to access information on immune cell markers, differentiation factors, cytokines, technical protocols, media recommendations, how-to videos, and more.
Positive or Negative Selection of CD3+ Pan T Cells, Which to Choose?
The difference between positive and negative cell selection comes down to how the CD3+ T cells are isolated. Positive selection means the target cell type of interest is actually "picked" from a population of cells using immunomagnetic beads with antibodies specific to surface biomarkers on the cells of interest. Negative selection means every other cell type which is not desired is removed using the immunomagnetic beads, thus leaving only the targeted cell type untouched.
| CD3+ T Cells | Advantages | Disadvantages |
| Positive Selection | • Better selection specificity • Higher CD3+ T cell yields | • Antibodies or labeling agents attached to target cells can impact subsequent applications/assays • Attached antibodies can lead to CD3+ T cell activation |
| Negative Selection | • Preferred when target cells have weak or unidentified surface biomarkers • CD3+ T cells are untouched with the immunomagnetic beads, thus avoiding unwanted cell activation | • May result in lower CD3+ T cell yields • Can be costly as various antibodies are needed for isolation |
What is the Percentage of CD3+ T Cells in a Leukopak?
To characterize T cell yields and assess inter-donor variability in leukapheresis collections, Charles River analyzed a cohort of 262 healthy donor fresh leukopak collections by examining the frequency of CD3+ T cells through flow cytometry. As shown in the figure below, the percentage of CD3+ T cells ranged between 21 – 77%, with an average of 48% of the total white blood cell (WBC) population in the leukopaks.
Frequently Asked Questions (FAQs) About CD3+ T Cells
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What biosafety measures should I consider when handling the CD3+ T cells?
Cells should be handled following biosafety level 2 procedures. Universal precautions should be used when working CD3+ cells. Use aseptic techniques when thawing or handling the cells.
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What are the storing and thawing instructions for CD3+ T cells?
Upon receipt, either prepare cells for long-term storage in liquid nitrogen vapor phase or thaw for immediate use. Storage in liquid phase nitrogen is NOT recommended. Long-term storage of cells at -80°C is NOT recommended. Once thawed, samples must be used immediately.
Video: Detailed T cell thawing instructions
Protocol Sheet: Step-by-step thawing instructions
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Which donor screening tests are performed for CD3+ T cells?
CD3+ T cells collected from donors are tested the same day in accordance with FDA requirements for blood for transfusion according to current FDA requirements for allogeneic blood donation. The following screening tests are performed and reported in the COA:
- Serological test for Syphilis
- Unexpected red cell antibody screening
- Hepatitis B Core Antibody (Anti-HBc EIA)
- Hepatitis B Surface Antigen (HBsAg EIA)
- Hepatitis C Virus Antibody (Anti-HCV EIA)
- Human Immunodeficiency Virus Antibody (HIV 1/2 plus O)
- Human T-Lymphotropic Virus Antibody (HTLV-I/II)
- HIV-1/HCV/HBV Nucleic Acid Testing
- West Nile Virus Nucleic Acid Testing
- Trypanosoma cruzi Antibody (Chagas Disease)
CMV testing result is also available upon request.