Features of Our CD4+ T Helper Cells
We perform rigorous donor screening and QC testing to ensure consistent CD4+ T cell lots for discovery and therapeutic development:
- Guaranteed post-thaw viability ≥ 90% as tested by NucleoCounter NC-200™
- Guaranteed post-thaw purity ≥ 90% as tested via flow cytometry
- Guaranteed CD4+ T helper cell yields post thaw as listed per vial size
- Isolated from fresh leukopaks collected from healthy human donors that have consented under an IRB approved protocol
- Available using positive or negative immunomagnetic cell isolation options
- Fill volume of 1ml of CD4+ TH cells in a 2ml vial size
- Comprehensive donor screening (serology and infectious disease panel)
Buy the CD4+ Helper T Cells that Best Suit Your Project Needs
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CD4+ Helper T Cells
CD4+ T Cells, (also known as T helper cells, TH cells, or helper T cells), are critical components of the adaptive immune system, regulating effective response to pathogens. CD4+ T Helper cells activate B cells to secrete antibodies, macrophages to eliminate harmful microorganisms, and cytotoxic T cells to kill infected cells.
Cell & Isolation Type Size Product Code CD4+ Helper T Cells
(Positive Selection)10 x 10e6 cells PB04C-1 25 x 10e6 cells PB04C-2 Custom PB04C-KIT* Full Leukopak Isolation PB04C-FL-KIT* CD4+ Helper T Cells
(Negative Selection)10 x 10e6 cells PB04NC-1 25 x 10e6 cells PB04NC-2 Custom PB04NC-KIT* Full Leukopak Isolation PB04NC-FL-KIT* Contact us if you don’t see a specific product, size, or format, or would like to customize your request.
* Product is made to order.Your company or academic institution may be eligible for pricing discounts. Government labs located in the United States are also eligible for GSA MAS discounts. Request a quote to find out more.
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Naive CD4+ T Cells
Naive CD4+ T cells are considered immature T cells that have not yet been activated through interactions with antigens. Naive CD4+ T cells are known to suppress the immune response in cases of chronic infection.
Cell & Isolation Type Size Product Code Naive CD4+ Helper T Cells
(Positive Selection)• 5 x 10e6 cells • PB0445RAC-1* Naive CD4+ Helper T Cells
(NegativeSelection)• 5 x 10e6 cells
• Full Leukopak Isolation• PB0445RANC-1*
• PB0445RANC-FL-KIT*Contact us if you don’t see a specific product, size, or format, or would like to customize your request.
* Product is made to order.Your company or academic institution may be eligible for pricing discounts. Government labs located in the United States are also eligible for GSA MAS discounts. Request a quote to find out more.
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Memory CD4+ T Helper Cells
Memory CD4+ T cells are T cells that have already encountered and been activated by specific antigens, and therefore can quickly form large numbers of effector T cells upon re-exposure to these invading antigens. Memory CD4+ T cells lie dormant in tissues and protect the body from the re-emergence of potentially pathogenic or harmful cells by providing a rapid response to past infection.
Cell & Isolation Type Size Product Code Memory CD4+ Helper T Cells
(Negative Selection)10 x 10e6 cells PB0445RONC-1* Full Leukopak Isolation PB0445RONC-FL-KIT* Contact us if you don’t see a specific product, size, or format, or would like to customize your request.
* Product is made to order.Your company or academic institution may be eligible for pricing discounts. Government labs located in the United States are also eligible for GSA MAS discounts. Request a quote to find out more.
What Are CD4+ T Cells Used for?
CD4+ TH cells play a critical role in regulating the immune system in response to pathogens and infections by mediating the activity of B cells, macrophages, and cytotoxic T cells to eliminate infection.
CD4+ helper T cells have been used in the development of effective immunotherapies (CAR-T) to induce remission in cancer patients, and many studies are underway to explore this anti-tumor response driven by the CD4+ T cells1.
CD4+ T cells can also be used as effective tools in the discovery and development of vaccines capable of stimulating durable protection against challenging viral infections such as HIV. CD4+ T cells have become an attractive target for therapeutic interventions against HIV pathogenesis. Recent scientific advancements involve genetically modifying CD4+ T cells to enhance their resistance to HIV infection or equipping them with HIV-specific receptors to enhance viral recognition and clearance. These innovative approaches using CD4+ helper T cells hold the potential to revolutionize HIV treatment2.
1. Kohler, M.E., Fry, T.J. CD4+ CAR T cells — more than helpers. Nat Cancer 4, 928–929 (2023).
2. Ron M. Significance and Applications of CD4 Cell Therapy and Immune System for HIV Infection. HIV Curr Res. 8:229 (2023).
Lineage of CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs)
Receive a complimentary poster to access information on immune cell markers, differentiation factors, cytokines, technical protocols, media recommendations, how-to videos, and more.
What is the Difference Between Positive and Negative Selection When Isolating CD4+ Helper T Cells?
CD4+ T cells are isolated using either positive or negative immunomagnetic cell separation techniques.
- During positive selection, the helper T cells are directly targeted by the immunomagnetic beads using antibodies specific to the CD4 surface biomarker.
- Negative selection means that every unwanted cell type is targeted by the antibodies on the immunomagnetic beads and is removed from the cell population, thus leaving only the desired CD4+ T cells untouched by immunomagnetic beads.
| CD4+ T Cells | Advantage | Disadvantage |
| Positive Selection | • Better selection specificity
| • Antibodies or labeling agents attached to target cells can impact subsequent applications/assays • Attached antibodies can lead to T cell activation |
| Negative Selection | • Preferred when target cells have weak or unidentified surface biomarkers • CD4+ T cells are untouched with the immunomagnetic beads, thus avoiding unwanted cell activation | • May result in lower CD4+ T cell yields
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What is the Percentage of CD4+ T Cells in a Leukopak?
To characterize T cell yields and assess inter-donor variability, Charles River analyzed a cohort of 263 healthy donor fresh leukopak collections by examining the frequency of CD4+ T cells through flow cytometry. As shown below, the percentage of CD4+ T cells ranged between 10 – 48%, with an average of 30% of the total white blood cell (WBC) population in the leukopaks.
Frequently Asked Questions About CD4+ T Helper Cells
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How are the CD4+ T cells cryopreserved?
Our CD4+ T cells are cryopreserved using CryoStor® CS10 pre-formulated with 10% DMSO, and then stored in the vapor phase of liquid nitrogen prior to shipping.
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What are the thawing and storage recommendations for the CD4+ T cells?
Upon receipt, either prepare the CD4+ TH cells for long-term storage in liquid nitrogen vapor phase or thaw for immediate use.
Storage in liquid phase nitrogen is NOT recommended. Long-term storage of cells at -80°C is NOT recommended. Once thawed, samples must be used immediately.
Please refer to this protocol for detailed step-by-step thawing instructions.
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What are biosafety considerations when handling the CD4+ T helper cells?
CD4+ T cells should be handled following biosafety level 2 procedures. Universal precautions should be used when working with these cells. Use aseptic techniques when thawing or handling the CD4+ T helper cells.
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Which screening tests are performed on the donors before isolation of the for the CD4+ TH cells?
CD4+ TH cells collected from donors are tested the same day in accordance with FDA requirements for blood for transfusion according to current FDA requirements for allogeneic blood donation. The following screening tests are performed and reported in the COA:
- Serological test for Syphilis
- Unexpected red cell antibody screening
- Hepatitis B Core Antibody (Anti-HBc EIA)
- Hepatitis B Surface Antigen (HBsAg EIA)
- Hepatitis C Virus Antibody (Anti-HCV EIA)
- Human Immunodeficiency Virus Antibody (HIV 1/2 plus O)
- Human T-Lymphotropic Virus Antibody (HTLV-I/II)
- HIV-1/HCV/HBV Nucleic Acid Testing
- West Nile Virus Nucleic Acid Testing
- Trypanosoma cruzi Antibody (Chagas Disease)
CMV testing result is also available upon request.
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How soon can I expect to receive my shipment of CD4+ T cells?
We will ship based on the shipping terms referenced in the Product Order Form (POF) when you place your order and after review and confirmation of your order.
Domestic Orders / Next-day Delivery
• Via specialty couriers
• Via FedEx First Overnight by 8:30 AM
• Via FedEx Priority by 10:30 AM
International Orders
Shipping times will vary depending on the destination country and the customs clearance process.