In vivo models engrafted with human immune cells are critical for immuno-oncology, infectious disease, and graft rejection research. Peripheral blood mononuclear cells (PBMCs) are of particular interest when humanizing mice because of their ability to quickly engraft in immunodeficient mice. The majority of mature human immune cells, when using PBMCs, are of human T cell origin (CD4+ and CD8+) within a week to 10 days.
Combined with our triple-immunodeficient NCG mouse model (strain code 572; lacking functional/mature T, B, and NK cells, along with reduced macrophage and dendritic cell function), pre-qualified PBMCs obtained from HemaCare (a Charles River Company) allows researchers to quickly confirm results, without the need to qualify donor cells.
This humanized PBMC (hu-PBMC) mouse model is ideal for short-term studies requiring a strong effector and memory T-cell and NK-cell function.
The HuPBMC-NCG mouse model is currently available in North America only.
Benefits of the HuPBMC-NCG Mouse Model
- Convenient – Ready-to-use PBMCs are pre-injected and tested for effective engraftment in the NCG mouse model.
- Efficient – Pre-screened PBMCs save time, labor, and costs associated with donor qualification. There are no license requirements.
- Quality – PBMC cell inventory has been screened for engraftment rate, body weight loss, and study term. Cell numbers have been optimized for use in the NCG mouse model.
- Trusted source – The engrafted NCG mouse is a result of a partnership between industry leaders with more than 100 years of combined experience in providing high-quality animal models and human biologics for research purposes.
Pricing and Ordering Information
Frequently Asked Questions (FAQs)
What is the timeline of PBMC injection vs. shipping?
Animals will be shipped 3 – 5 business days after the injection of the cells.
What is the optimal age range for the NCG mice to be injected with PBMCs?
Generally, injection of the cells occurs in animals between 4 – 8 weeks of age, but timing may be adjusted to meet your study needs. Once an order is confirmed, we will provide the ages of available animals and the shipment date of each order.
How many PBMCs are injected into the NCG mice?
Approximately 10 x 106 cells are injected per mouse.
Can I request a specific lot of cells to be used for my order?
Please contact Client Relations at 1-800-522-7287 if you would like to pre-order/pay for a specific lot of cells.
Is there a minimum number of HuPBMC-NCG mice per order?
The minimum is 5 animals per order. Additional quantities may be ordered in increments of 5 animals.
When should the HuPBMC-NCG mice be placed on study?
The ideal study start time for HuPBMC-NCG mice is between 21 – 45 days post-injection, prior to the onset of graft-versus-host disease (GvHD).
What is the general turnaround time for the animals to ship after I place my order?
It can take 2 – 3 weeks for the full process.