Embryo & Sperm Cryopreservation

Charles River can back up your live animal colony or stored line with low-cost embryo and sperm cryopreservation services. These services help guard against genetic drift and prevent loss due to disease, ensuring that your strains will be there when you need them.

To protect your cryopreserved embryos and sperm, they will be divided between two independent locations, each with its own automated liquid nitrogen filling system and alarm network.

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Service Description Requirements Deliverables
Embryo Cryopreservation For mouse and rat strains Mice:
  • 8-10 breeder males: 12 weeks-6 months old
  • 20 females: 3-4 weeks old
  • 8-10 males: < 6 months old
  • 20 females: 8-10 weeks old
  • 250-300 embryos (heterozygous lines)
  • 150-200 embryos (homozygous lines)
Cryopreservation with HO Breeding Homozygous strain undergoes preliminary expansion breeding 5 breeding pairs
  • 150-200 embryos
  • Homozygous expansion breeding
Sperm Cryopreservation Cryopreservation of sperm from 2 males
* Mice only
2 breeder males: 12 weeks-6 months old
  • Pre- and post-thaw QC
  • 15 straws preserved, most strains
Germplasm Cryostorage Secure cryostorage in two independent facilities