Creating and Maintaining Customized Knockin Mouse Models
The ability to engineer the mouse genome has made transgenic mice a powerful tool for modeling human disease. Together with our industry partners, all types of mutations are achievable: complex and mutli-allelic mutations, constitutive knockin mice, humanized mice, point mutation knockin mice, tag/reporter, and target transgenesis.
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Knockin Mice Mutations
- Constitutive KI Mice: This model is engineered to carry a cDNA sequence coding for a protein in a selected locus.
- Point Mutation KI Mice: This model consists of the replacement of one or a few DNA bases in the sequences of a specified gene.
- Humanized Mice: This model consists of the replacements of the murine gene by its human counterpart.
Frequently Asked Questions (FAQs) About Knockin Mice
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What are some of the advantages of using knockin mouse models?
Knockin mice are used for multiple applications, such as human disease recapitulation, compound efficacy assessment, promoter activity study, mutant protein expression, cell trafficking following marker gene introduction, gene replacement by reporter gene, and humanization.
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Are there further considerations for knockin mouse projects?
Modification of human orthologue proteins in mice may not completely reflect the role of a gene in human pathology.
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What are the methods used for generating a knockin mouse model?
Depending on your project and objectives, knockin mice can be obtained through homologous recombination in ES cells, genome editing nucleases, random transgenesis, and targeted transgenesis.
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What further services do you offer for knockin mice?
- Mouse & Rat Contract Breeding
- Transgenic Mouse & Rat Model Creation
- Genetic Testing Services
- Embryology (including cryopreservation and rederivation)
- Rapid Colony Development
- Animal Quarantine
- Line Rescue