Many acute toxicity tests have been or are being effectively replaced by in vitro human, animal and 3D tissue alternatives. This shift is particularly evident in skin irritation, skin corrosion and skin sensitization models, all of which are fully supported at Charles River.
For topically applied or exposed chemicals and drugs, assessment of the skin corrosion and skin irritation potential is a key element in their safety evaluations. Irritation and corrosion testing uses 3D human skin models (EpiDerm™ and EpiSkin®). In addition, an in chemico assay, Corrositex®, can be used to assign skin corrosion classifications. In silico models are also used to identify skin corrosion, irritation or sensitization potential.
Skin sensitization is a hypersensitivity reaction resulting from the interaction of a sensitizing agent with host proteins to form an immunogenic complex. Examples of sensitizers include para-phenylenediamine (PPD) in hair dyes and nickel in clothes or jewelry. When these small molecules (haptens) bind to proteins, they become immunogenic and activate the immune system, which causes localized inflammation at the site of sensitizing agent exposure.
There is now a regulatory requirement for REACH testing to utilize these alternative models in a tiered approach ahead of animal tests. Many of these tests are also applicable to screening prior to in vivo testing.
As part of our integrated toxicology testing strategy, there may be cases where the in vivo test should be performed rather than the in vitro test. For example, the in vivo test should be used when a classification cannot be assigned or where a regulatory authority specifically requires in vivo data. In European labs, in vitro tests and data collection must be performed prior to evaluating the need for or performing in vivo testing.
- In vitro Percutaneous Absorption and Metabolism of Bisphenol A (BPA) Through Fresh Human Skin
- Application of In Vitro Skin Penetration Measurements to Confirm and Refine the Quantitative Skin Sensitization Risk Assessment of Methylisothiazolinone
- DPRA (direct peptide reactivity assay)
- KeratinoSens and LuSens
- In silico (QSAR)
- LLNA (local lymph node assay)
- GPMT (guinea pig maximization test)
- Buehler assay (contact sensitization)
- DTH (delayed-type hypersensitization)
- SkinEthic EpiSkin® skin irritation (OECD 439)
- MatTek EpiOcular® eye irritation (OECD 492)
- Bovine cornea and opacity permeability (BCOP – OECD 437)
- Hen’s Egg Test (HET)-CAM ocular irritation
- Ocular and dermal acute non-rodent
- Acute dermal rodent
- SkinEthic EpiSkin® skin corrosion (OECD 431)
- Corrositex® skin corrosion (OECD 435)
- Acute dermal rodent